Under the standards, enter the test sample absorbance values, leaving their concentration values blank.
Note, the absorbance values I have entered into GraphPad have already been background-corrected. Thus, simply remove the background absorbance value from all of the samples, including the controls. There is no need to include the blank/background sample in the GraphPad sheet. The image below shows what the data sheet looks like for this example. The standards are in rows 1 – 7 and the test samples are in the rows after this. Now we are ready to interpolate the standard curve using GraphPad. To do this, simply follow the steps below.ġ. With the data sheet open, find the ‘ Analysis‘ ribbon at the top and click the ‘ Interpolate a standard curve‘ button.
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